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教育訓練課程

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Translational Research: How NanoString complements NGS (in English)

  • 日期: 10 月 29 日 二
    10:00~12:00
  • 主講人: Dr.Michael Rhodes (Director of applications development)
  • 地點:生醫所B1B會議室
This lecture is hosted by Cold Spring Biotech Crop.

Next Generation Sequencing ahas revolutionised the discovery in the biological sciences. The challenge becomes taking these discoveries through the translational research pipeline. The NanoString nCounter system offers a solution for this, especially for gene expression studies of a panel of genes (up to 800) following and RNA-Seq experiment. The NanoString nCounter system is a novel platform for direct digital quantification of nucleic acids, including difficult samples such as single cells, tissue, and blood lysates, as well as RNA and DNA from FFPE tissues. The multiplexing capacity of 10s to as many as 800 different genes / targets in a single reaction facilitates screening of complex signatures, pathways, or gene classes with incredible simplicity and precision. The technology works by directly capturing, imaging, and counting fluorescent barcodes tagged to individual nucleic acid molecules. This is done without amplification or reverse transcription (resulting in succesfull analysis of lysates and degraded samples). The result is an assay capable of sensitive and precise quantification of mRNA, miRNA, or DNA (for CNV or ChIP screening), as well as the characterization of gene fusions, splice variants, and even microbial populations.

An introduction to the assay chemistry and performance will be provided, followed by specific examples of the application of the technology for tumor stratification and de-convolution of complex gene networks will be discussed.

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研討會資訊

主講者:Dr. Philip N. Benfey (美國國家科學院院士、美國杜克大學特聘教授)
講 題:A Systems Approach to Improved Root Traits
時 間:2013年10月22日(星期二) 上午11時
地 點:本院分生所地下室演講廳
主講者:Dr. Tuan-Hua David Ho
講題:An ortholog of the Arabidopsis flowering gene FCA actually regulates seed germination in rice
時間:2013年10月25日(星期五) 上午11時
地點:本院植微所農科大樓 A134會議室
講題:Synaptic plasticity in learning and momory
主講者:Dr. Yu Tian Wang (Univ. of British Columbia, Vancouver)
時間:2013年10月28日(星期一) 上午10時-11時
地點:Academia Sinica (B1B Lecture Room, Inst. of Biomedical Sciences)
會議日期:中華民國102年11月4日至11月5日
主辦單位:中央研究院生物化學研究所
會議地點:中央研究院人文社會科學館第一會議室
會議日期:中華民國102年11月8日至11月9日
主辦單位:中央研究院生物多樣性研究中心
合辦單位:台灣演化與計算生物學會、國科會生命科學研究推動中心、國立中興大學基因體暨生物資訊學研究所、中央研究院資訊科學研究所、台灣大學生命科學院
會議地點:國立中興大學 管理學院國際會議廳

English version-newsletter

New talks are added in October, please access it
Training Courses Materials Updates, please access it!
This course is held on October 24, welcome to register it!
This course is held on October 29, welcome to register it!
Welcome to MicrobeLibrary! Please access it!
Venue:Conference Room, College of Management, National Chung Hsing University
Time: 8-9 November, 2013
Venue:1st conference room, Research Center for Humanities and Social Sciences
Time: 4-5 November, 2013