{"id":1032,"date":"2017-06-19T15:23:37","date_gmt":"2017-06-19T07:23:37","guid":{"rendered":"https:\/\/lsl.sinica.edu.tw\/Blog\/?p=1032"},"modified":"2017-08-17T11:38:58","modified_gmt":"2017-08-17T03:38:58","slug":"searchgui","status":"publish","type":"post","link":"https:\/\/lsl.sinica.edu.tw\/Blog\/2017\/06\/searchgui\/","title":{"rendered":"\u4f7f\u7528SearchGUI\u548cPeptideShaker\u5b9a\u6027\u8cea\u8b5c\u5100\u86cb\u767d\u9ad4\u8cc7\u6599"},"content":{"rendered":"<h3><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/20170619.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-1026\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/20170619-768x1024.jpg\" alt=\"20170619\" width=\"580\" height=\"773\" \/><\/a><\/h3>\n<h3><\/h3>\n<p><!--more--><\/p>\n<h3>\u8cea\u8b5c\u5100\u662f\u5206\u6790\u86cb\u767d\u9ad4(Proteome)\u8868\u73fe\u5f88\u91cd\u8981\u7684\u65b9\u6cd5\u4e4b\u4e00\uff0c\u4e86\u89e3\u5206\u6790\u6a23\u54c1\u4e2d\u542b\u6709\u90a3\u4e9b\u86cb\u767d\u8cea\u901a\u5e38\u662f\u5206\u6790\u7684\u7b2c\u4e00\u6b65\u3002\u76ee\u524d\u6709\u8a31\u591a\u86cb\u767d\u8cea\u641c\u5c0b\u5f15\u64ce\u53ef\u4ee5\u5e6b\u6211\u5011\u4f86\u4e86\u89e3LC-MS\/MS\u8cc7\u6599\uff0c\u4f5c\u86cb\u767d\u8cea\u5b9a\u6027\u5206\u6790(Protein Identification)\uff0c\u7136\u800c\u4e0d\u540c\u7684\u641c\u5c0b\u5f15\u64ce\u6709\u8457\u4e0d\u540c\u7684\u8a08\u7b97\u65b9\u6cd5\uff0c\u5b9a\u6027\u7684\u7d50\u679c\u53ef\u80fd\u4e0d\u76e1\u76f8\u540c\u3002\u6bd4\u5229\u6642Lennart Martens\u6559\u6388\u7684\u7814\u7a76\u5718\u968a\u958b\u767c\u4e86SearchGUI\u8edf\u9ad4\uff0c\u6574\u5408\u4e868\u5957\u86cb\u767d\u8cea\u641c\u5c0b\u5f15\u64ce\uff0c\u900f\u904ePeptideShaker\u4f86\u5448\u73fe\u6574\u5408\u5f8c\u7684\u641c\u5c0b\u7d50\u679c\uff0c\u4e26\u652f\u63f4\u8de8\u5e73\u53f0\uff0c\u64cd\u4f5c\u4ecb\u9762\u76f4\u89ba\uff0c\u76f8\u7576\u65b9\u4fbf\u3002<\/h3>\n<p>&nbsp;<\/p>\n<h3>\u9996\u5148\u5230\u4e0b\u5217\u7db2\u5740\u4e0b\u8f09SearchGUI\u548cPeptideShaker\u3002<\/h3>\n<h3><a href=\"https:\/\/compomics.com\/bioinformatics-for-proteomics\/\">https:\/\/compomics.com\/bioinformatics-for-proteomics\/<\/a><\/h3>\n<h3><a href=\"https:\/\/compomics.github.io\/projects\/peptide-shaker.html\">https:\/\/compomics.github.io\/projects\/peptide-shaker.html<\/a><\/h3>\n<h3><\/h3>\n<h3>\u5b89\u88dd\u5f8c\uff0c\u958b\u555fSearchGUI\u53ef\u4ee5\u770b\u5230\u64cd\u4f5c\u4ecb\u9762\u5982\u4e0b\u5716\u3002<\/h3>\n<h3>Input &amp; Output\u4e2d\u9078\u64c7\u8cea\u8b5c\u8cc7\u6599\u7684\u6a94\u6848\u3001\u7de8\u8f2f\u641c\u5c0b\u8a2d\u5b9a\u548c\u6307\u5b9a\u8f38\u51fa\u7d50\u679c\u5b58\u653e\u7684\u4f4d\u7f6e\u3002<\/h3>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome1.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1027\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome1.png\" alt=\"Proteome1\" width=\"906\" height=\"859\" \/><\/a><\/p>\n<h3>\u5728\u641c\u5c0b\u8a2d\u5b9a\u7de8\u8f2f\u7684\u9801\u9762\uff0c\u53ef\u4ee5\u9078\u64c7FASTA\u6a94\u3001\u5b9a\u7fa9Modification\u7684\u7a2e\u985e\u3001\u7de8\u8f2f\u641c\u5c0b\u53c3\u6578\u7b49\u7b49\u3002<\/h3>\n<h3><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1028\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome2.png\" alt=\"Proteome2\" width=\"902\" height=\"744\" \/><\/a><\/h3>\n<h3>\u8a2d\u5b9a\u5b8c\u6210\u5f8c\uff0c\u9078\u64c7\u8981\u57f7\u884c\u7684\u641c\u5c0b\u5f15\u64ce\uff0c\u8a18\u5f97\u4e5f\u9078\u53d6PeptideShaker\uff0c\u9019\u6a23SearchGUI\u57f7\u884c\u5b8c\u7562\u5f8c\u6703\u76f4\u63a5\u5c07\u7d50\u679c\u5448\u73fe\u5728PeptideShaker\u3002\u9078\u597d\u5f8c\uff0c\u6309\u4e0b\u300cSearch the Search!\u300d\uff0c\u958b\u59cb\u57f7\u884c\u3002<\/h3>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome3.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1029\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome3.png\" alt=\"Proteome3\" width=\"910\" height=\"654\" \/><\/a><\/p>\n<h3>\u8dd1\u5b8c\u5f8c\uff0cPeptideShaker\u5728Overview\u7684\u5206\u9801\u5448\u73feIdentified Proteins\u3001\u9078\u64c7\u7684Identified Protein\u662f\u7d93\u7531\u90a3\u4e9bPeptides\u7d44\u6210\u3001Peptide\u7684PSMs(Peptide Spectrum Matchs)\u662f\u90a3\u4e9b\u8207\u5176\u5c0d\u61c9\u7684\u5716\u8b5c\u3002<\/h3>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome4.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-1030\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome4-1024x552.png\" alt=\"Proteome4\" width=\"580\" height=\"312\" \/><\/a><\/p>\n<h3>\u9664\u4e86Overview\uff0cPeptideShaker\u9084\u63d0\u4f9b\u5f88\u591a\u597d\u7528\u7684\u5206\u6790\u529f\u80fd\uff0c\u5305\u62ecSpectrum IDs\u3001Fractions\u3001Modifications\u30013D Structures\u3001Annotation\u3001GO Analysis\u3001Validation\u548cQC Plots\u3002PeptideShaker\u7684\u4f7f\u7528\u8aaa\u660e\u5f88\u8c50\u5bcc\uff0c\u4f7f\u7528\u8005\u53ef\u4ee5\u81f3\u5b98\u65b9\u7db2\u7ad9\u67e5\u8a62\u8a73\u7d30\u7684\u529f\u80fd\u3002<\/h3>\n<h3><a href=\"https:\/\/compomics.com\/bioinformatics-for-proteomics\/\">https:\/\/compomics.com\/bioinformatics-for-proteomics\/<\/a><\/h3>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome5.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-1031\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2017\/06\/Proteome5-1024x554.png\" alt=\"Proteome5\" width=\"580\" height=\"313\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h1 style=\"text-align: center;\"><strong><span style=\"color: #0000ff;\">\u611f\u8b1d TIGP \u7684\u00a0Wayne \u63d0\u4f9b\u4ee5\u4e0a\u8cc7\u6599<\/span><\/strong><\/h1>\n<div class=\"pld-like-dislike-wrap pld-template-1\">\r\n    <div class=\"pld-like-wrap  pld-common-wrap\">\r\n    <a href=\"javascript:void(0)\" class=\"pld-like-trigger pld-like-dislike-trigger  \" title=\"\u8b9a\" data-post-id=\"1032\" data-trigger-type=\"like\" data-restriction=\"ip\" data-already-liked=\"0\">\r\n                        <i class=\"fas fa-thumbs-up\"><\/i>\r\n                <\/a>\r\n    <span class=\"pld-like-count-wrap pld-count-wrap\">0    <\/span>\r\n<\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>0<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18,8],"tags":[9,16,58,19,57],"class_list":["post-1032","post","type-post","status-publish","format-standard","hentry","category-bioinformatics","category-databases","tag-database","tag-dr-a-cula-lab","tag-peptideshaker","tag-protein","tag-searchgui"],"_links":{"self":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/1032","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/comments?post=1032"}],"version-history":[{"count":9,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/1032\/revisions"}],"predecessor-version":[{"id":1132,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/1032\/revisions\/1132"}],"wp:attachment":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/media?parent=1032"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/categories?post=1032"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/tags?post=1032"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}