{"id":420,"date":"2015-08-03T15:08:17","date_gmt":"2015-08-03T07:08:17","guid":{"rendered":"https:\/\/lsl.sinica.edu.tw\/Blog\/?p=420"},"modified":"2015-09-01T10:23:09","modified_gmt":"2015-09-01T02:23:09","slug":"hho-1","status":"publish","type":"post","link":"https:\/\/lsl.sinica.edu.tw\/Blog\/2015\/08\/hho-1\/","title":{"rendered":"\u904b\u7528\u751f\u7269\u8cc7\u8a0a\u5de5\u5177\u9810\u6e2c\u86cb\u767d\u8cea\u4e8c\u7d1a\u7d50\u69cb\u53ca\u5206\u6790\u7d50\u69cb\u7279\u6027"},"content":{"rendered":"<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/ChatRoom_20150803.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-424\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/ChatRoom_20150803.jpg\" alt=\"ChatRoom_20150803\" width=\"848\" height=\"1000\" \/><\/a><\/p>\n<p><!--more--><\/p>\n<p>&nbsp;<\/p>\n<h3><strong>\u7bc4\u4f8b<\/strong>\uff1ahHO-1(human heme oxygenase-1) \u4eba\u985e\u8840\u7d05\u7d20\u52a0\u6c27\u9176-1<\/h3>\n<h3>1. \u5f9e\u5be6\u9a57\u4e2d\u767c\u73fehHO-1\u6709\u4e8c\u805a\u9ad4\u7684\u5f62\u6210\uff0c\u800c\u4e14\u9019\u4e9b\u4e8c\u805a\u9ad4\u50c5\u50c5\u5728\u86cb\u767d\u8cea\u5168\u93c8\u6642\u624d\u80fd\u5920\u88ab\u6e2c\u5f97\u3002\u56e0\u6b64\u7d66\u4e88\u4ee5\u4e0b\u7684\u5047\u8a2d\uff0chHO-1\u5ea7\u843d\u5728\u5167\u8cea\u7db2 (endoplasmic reticulum; ER) \u7684\u7db2\u819c\u4e0a\u662f\u5229\u7528\u86cb\u767d\u8cea\u5e8f\u5217\u7684C\u7aef(The C-terminal region)\u7684\u8de8\u819c\u7d50\u69cb\u5340\u6bb5 (transmembrane segment; TMS)\uff0c\u56e0\u6b64\u6700\u6709\u53ef\u80fd\u767c\u751f\u4e8c\u805a\u9ad4\u7684\u4ecb\u9762 (dimeric interface) \u662f\u5728\u6b64\u4e00\u8de8\u819c\u7d50\u69cb\u5340\u6bb5\u4e0a\u3002\u5982\u679c\u8981\u8b49\u660e\u9019\u500b\u5047\u8a2d\uff0c\u5c31\u8981\u9032\u884c\u80fa\u57fa\u9178\u7684\u55ae\u9ede\u7a81\u8b8a\u5be6\u9a57\uff0c\u53ef\u662f\u8981\u5728\u54ea\u4e00\u500b\u6216\u54ea\u4e9b\u80fa\u57fa\u9178\u9032\u884c\u55ae\u9ede\u7a81\u8b8a\u5462\uff1f<\/h3>\n<p>&nbsp;<\/p>\n<h3>2. \u641c\u5c0b\u86cb\u767d\u8cea\u7d50\u69cb\u8cc7\u6599\u5eab ( Protein Data Bank; PDB; http:\/\/www.rcsb.org\/pdb\/home\/home.do)\u3000\u5f8c\uff0chHO-1(\u5168\u9577288\u80fa\u57fa\u9178)\u7684\u4e09\u7d1a\u7d50\u69cb\u4e0d\u5305\u62ecC\u7aef\u7684\u7247\u65b7 (1-223\u80fa\u57fa\u9178)<\/h3>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/20150803-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-421\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/20150803-1.jpg\" alt=\"20150803-1\" width=\"250\" height=\"250\" \/><\/a><\/p>\n<h3><\/h3>\n<h3>3. \u9810\u6e2c\u86cb\u767d\u8cea\u4e8c\u7d1a\u7d50\u69cb\u548c\u8de8\u819ca\u87ba\u65cb (a-helix) \u7d50\u69cb\u4f86\u6c7a\u5b9a\u8de8\u819c\u5340\u6bb5\u7684\u53ef\u80fd\u7684\u7bc4\u570d\uff0c\u53ef\u4ee5\u904b\u7528\u7684\u5de5\u5177\u6709\uff1a<\/h3>\n<table>\n<tbody>\n<tr>\n<td width=\"254\">\n<h3 style=\"text-align: center;\">\u9810\u6e2c\u4e8c\u7d1a\u7d50\u69cb<\/h3>\n<\/td>\n<td width=\"516\">\n<h3 style=\"text-align: center;\">\u7db2\u5740<\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"254\">\n<h3>PredictProtein<\/h3>\n<\/td>\n<td width=\"516\">\n<h3><a href=\"https:\/\/www.predictprotein.org\/\">https:\/\/www.predictprotein.org\/<\/a><\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"254\">\n<h3>PSIpred<\/h3>\n<\/td>\n<td width=\"516\">\n<h3><a href=\"http:\/\/bioinf.cs.ucl.ac.uk\/psipred\/\">http:\/\/bioinf.cs.ucl.ac.uk\/psipred\/<\/a><\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"254\">\n<h3>Scratch Protein Predictor<\/h3>\n<\/td>\n<td width=\"516\">\n<h3><a href=\"http:\/\/scratch.proteomics.ics.uci.edu\/index.html\">http:\/\/scratch.proteomics.ics.uci.edu\/index.html<\/a><\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"254\">\n<h3>PredictProtein<\/h3>\n<\/td>\n<td width=\"516\">\n<h3><a href=\"https:\/\/www.predictprotein.org\/\">https:\/\/www.predictprotein.org\/<\/a><\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"254\">\n<h3>PSIpred<\/h3>\n<\/td>\n<td width=\"516\">\n<h3><a href=\"http:\/\/bioinf.cs.ucl.ac.uk\/psipred\/\">http:\/\/bioinf.cs.ucl.ac.uk\/psipred\/<\/a><\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"254\">\n<h3>SOSUI<\/h3>\n<\/td>\n<td width=\"516\">\n<h3><a href=\"http:\/\/harrier.nagahama-i-bio.ac.jp\/sosui\/\">http:\/\/harrier.nagahama-i-bio.ac.jp\/sosui\/<\/a><\/h3>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h3>\u7d9c\u5408\u4ee5\u4e0a\u4e09\u7d1a\u7d50\u69cb\u53ca\u4e8c\u7d1a\u7d50\u69cb\u9810\u6e2c\u7684\u7d50\u679c\uff0c\u4ee5\u5716\u5f62\u4f86\u986f\u793ahHO-1\u7684\u4e8c\u7d1a\u7d50\u69cb\u5143\u7d20 (secondary structure elements)\uff0c\u9ed1\u8272\u7684\u5340\u57df\u662f\u63a8\u5c0e\u81ea\u4e09\u7d1a\u7d50\u69cb\u548c\u7070\u8272\u7684\u5340\u57df\u662fC\u7aef\u4f86\u81ea\u4e8c\u7d1a\u7d50\u69cb\u9810\u6e2c\uff0c\u8de8\u819c\u5340\u6bb5\u5247\u662f\u7d05\u8272\u7684\u5340\u57df\u3002<\/h3>\n<p>&nbsp;<\/p>\n<h3><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/20150803-2.gif\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-422\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/20150803-2.gif\" alt=\"20150803-2\" width=\"646\" height=\"319\" \/><\/a><\/h3>\n<h3><\/h3>\n<h3>4. \u9810\u6e2c\u8de8\u819c\u5340\u6bb5\u7684\u7d50\u69cb\u7279\u6027coiled-coil <em>a-g<\/em>\u4e03\u80bd\u91cd\u8907 (<em>a-g<\/em> heptad repeat)\u647a\u758a\u7684\u5206\u6790\u7684\u5de5\u5177\u6709\uff1a<\/h3>\n<table>\n<tbody>\n<tr>\n<td width=\"172\">\n<h3 style=\"text-align: center;\">\u9810\u6e2c\u5de5\u5177<\/h3>\n<\/td>\n<td width=\"553\">\n<h3 style=\"text-align: center;\">\u7db2\u5740<\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"172\">\n<h3>Coils<\/h3>\n<\/td>\n<td width=\"553\">\n<h3><a href=\"http:\/\/www.ch.embnet.org\/software\/COILS_form.html\">http:\/\/www.ch.embnet.org\/software\/COILS_form.html<\/a><\/h3>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"172\">\n<h3>Paircoil2<\/h3>\n<\/td>\n<td width=\"553\">\n<h3><a href=\"http:\/\/paircoil2.csail.mit.edu\/\">http:\/\/paircoil2.csail.mit.edu\/<\/a><\/h3>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><\/h3>\n<h3>\u5c07\u5206\u6790\u7684\u7d50\u679c\u4ee5\u87ba\u65cb\u8f2a (helicalwheel) \u5716\u5f62\u4f86\u6a19\u793a\uff0c\u5176\u4e2d\u4f4d\u7f6e<em>a<\/em> (position <em>a<\/em>)\u548c\u4f4d\u7f6e<em>d<\/em> (position <em>d<\/em>) \u5747\u5c6c\u758f\u6c34\u6027\u80fa\u57fa\u9178\uff0c\u56e0\u6b64\u5047\u8a2d\u9019\u4e00\u9762\u5411\u53ef\u80fd\u662f\u4e8c\u805a\u9ad4\u7684\u4ecb\u9762\u3002\u4f9d\u64da\u80fa\u57fa\u9178\u7d50\u69cb\u7279\u6027\u53ca\u53ef\u80fd\u4f4d\u65bc\u4e8c\u805a\u9ad4\u4ecb\u9762\u7684\u4e2d\u592e\u800c\u9078\u64c7Trp270\u70ba\u7a81\u8b8a\u9ede\u7684\u6a19\u7684\u3002<\/h3>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/20150803-3.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-423\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/20150803-3.jpg\" alt=\"20150803-3\" width=\"486\" height=\"374\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<h3>\u9019\u4e9b\u7d50\u679c\u767c\u8868\u5728\uff1a<\/h3>\n<h3>Oligomerization Is Crucial for the Stability and Function of Heme Oxygenase-1 in the Endoplasmic Reticulum. Hsuan-Wen Hwang, <em>et al<\/em>. J. Biol. Chem. 2009 284: 22672-22679.[<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=19556236\"><strong><span style=\"font-family: Thread-000014e8-Id-00000022;\">PubMed link<\/span><\/strong><\/a>]<\/h3>\n<h3><\/h3>\n<h3><span style=\"color: #0000ff;\"><strong><span style=\"font-family: Thread-00001b6c-Id-00000080;\"><span style=\"font-family: \u65b0\u7d30\u660e\u9ad4;\">\u4ee5\u4e0a\u5167\u5bb9\u611f\u8b1d\u751f\u91ab\u6240\u5b6b\u6176\u59dd\u5c0f\u59d0\u63d0\u4f9b\uff0c\u5e0c\u671b\u5c0d\u60a8\u5011\u6709\u5e6b\u52a9\u3002<\/span><\/span><\/strong><\/span><\/h3>\n<div class=\"pld-like-dislike-wrap pld-template-1\">\r\n    <div class=\"pld-like-wrap  pld-common-wrap\">\r\n    <a href=\"javascript:void(0)\" class=\"pld-like-trigger pld-like-dislike-trigger  \" title=\"\u8b9a\" data-post-id=\"420\" data-trigger-type=\"like\" data-restriction=\"ip\" data-already-liked=\"0\">\r\n                        <i class=\"fas fa-thumbs-up\"><\/i>\r\n                <\/a>\r\n    <span class=\"pld-like-count-wrap pld-count-wrap\">0    <\/span>\r\n<\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>0<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[16,19],"class_list":["post-420","post","type-post","status-publish","format-standard","hentry","category-bioinformatics","tag-dr-a-cula-lab","tag-protein"],"_links":{"self":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/420","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/comments?post=420"}],"version-history":[{"count":5,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/420\/revisions"}],"predecessor-version":[{"id":457,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/420\/revisions\/457"}],"wp:attachment":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/media?parent=420"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/categories?post=420"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/tags?post=420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}