{"id":449,"date":"2015-08-31T11:34:38","date_gmt":"2015-08-31T03:34:38","guid":{"rendered":"https:\/\/lsl.sinica.edu.tw\/Blog\/?p=449"},"modified":"2015-09-01T10:58:27","modified_gmt":"2015-09-01T02:58:27","slug":"fap","status":"publish","type":"post","link":"https:\/\/lsl.sinica.edu.tw\/Blog\/2015\/08\/fap\/","title":{"rendered":"\u6210\u7e96\u7dad\u7d30\u80de\u6d3b\u5316\u86cb\u767d (FAP; fibroblast activation protein)\u7684\u6f5b\u5728\u7684\u57fa\u8cea(substrate)"},"content":{"rendered":"<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/ChatRoom-20150831.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-455\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/ChatRoom-20150831.jpg\" alt=\"ChatRoom-20150831\" width=\"848\" height=\"1000\" \/><\/a><\/p>\n<p><!--more--><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h3>Purpose: \u5728\u86cb\u767d\u8cea\u5e8f\u5217\u9032\u884c\u5927\u898f\u6a21\u7684 (large scale) \u641c\u5c0b\u6f5b\u5728\u7684\u57fa\u8cea\u4e26\u4ee5\u6a21\u64ec\u4e09\u7dad\u7d50\u69cb (model of 3D structure)\u986f\u793a\u3002<\/h3>\n<h3>1. \u6839\u64da\u6587\u737b\u8cc7\u6599\u986f\u793a\uff0c\u6210\u7e96\u7dad\u7d30\u80de\u6d3b\u5316\u86cb\u767d\u7684\u57fa\u8cea\u5177\u6709\u86cb\u767d\u8cea\u5e8f\u5217\u5c08\u4e00\u6027 (sequence specificity) (<em>FEBS lett<\/em>, 2006, <strong>580<\/strong>:1581-1586)\uff0c\u57fa\u8cea\u7684P1\u4f4d\u7f6e\u70ba\u812f\u6c28\u9178 (Proline) \u548c P2\u4f4d\u7f6e\u70ba\u7518\u6c28\u9178 (Glycine).<\/h3>\n<h3>2. \u5f9e\u86cb\u767d\u8cea\u5e8f\u5217\u8cc7\u6599\u5eabUniProtKB (<a href=\"http:\/\/www.uniprot.org\/\">http:\/\/www.uniprot.org\/<\/a>)\u4e0b\u8f09\u86cb\u767d\u8cea\u5e8f\u5217\uff0c\u5e8f\u5217\u4e4b\u9593\u662f\u4ee5\u201c\/\/\u201d\u5340\u9694\u3002<\/h3>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/seq.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-452\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/seq.jpg\" alt=\"seq\" width=\"700\" height=\"854\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<h3>3. \u8a2d\u5b9a\u540d\u7a31\u201chuman\u201d \u3001\u201cextracellular\u201d\u3001\u201cmembrane bound\u201d\u3001\u201c secret\u201d\u3002\u5f9e\u4e2d\u641c\u5c0b\u8a2d\u5b9a\u540d\u7a31\uff0c\u518d\u5c07\u9078\u64c7\u7b26\u5408\u689d\u4ef6\u7684\u5e8f\u5217\u9032\u884c\u4ee5\u4e0b\u5b57\u4e32\u641c\u5c0b\u3002<\/h3>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/screening.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-459\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/screening.jpg\" alt=\"screening\" width=\"712\" height=\"445\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<h3>4. \u7b26\u5408\u5b57\u4e32\u641c\u5c0b\u4e26\u7d93\u904e\u86cb\u767d\u8cea\u4e09\u7d1a\u7d50\u69cb\u5206\u6790\u6216\u4e8c\u7d1a\u7d50\u69cb\u9810\u6e2c\uff0c\u4fc2\u4f4d\u65bc\u7d50\u69cb\u5f4e\u66f2\u90e8\u5206 (loop)\u7684\u500b\u6848\u5247\u70ba\u63a5\u4e0b\u4f86\u5be6\u9a57\u7684\u5c0d\u8c61\u3002\u5176\u4e2d SPRY2 (Protein sprouty homolog 2)\u88ab\u6210\u7e96\u7dad\u7d30\u80de\u6d3b\u5316\u86cb\u767d\u6240\u88c2\u89e3 (cleavage)\u3002<\/h3>\n<h3>5. \u5f9e\u86cb\u767d\u8cea\u7d50\u69cb\u8cc7\u6599\u5eab (PDB; <a href=\"http:\/\/www.rcsb.org\/pdb\/home\/home.do\">http:\/\/www.rcsb.org\/pdb\/home\/home.do<\/a>)\u4e0b\u8f09\u6210\u7e96\u7dad\u7d30\u80de\u6d3b\u5316\u86cb\u767d\u7684\u4e09\u7d1a\u7d50\u69cb (PDB code 1Z68 and 1NU8)\uff0c\u904b\u7528InsightII\u8edf\u9ad4 [InsightII \u70ba\u820a\u7248\u8edf\u9ad4\uff0c\u73fe\u5728\u4f7f\u7528\u7684\u662fBIOVIA Discovery Studio (<a href=\"http:\/\/accelrys.com\/products\/collaborative-science\/biovia-discovery-studio\/\">http:\/\/accelrys.com\/products\/collaborative-science\/biovia-discovery-studio\/<\/a>)] \u4ee5diprotin A\u7684\u7d50\u69cb\u70ba\u57fa\u790e\uff0c\u7522\u751fSPRY2\u7684SSGPVA\u7d50\u69cb\u7247\u6bb5\uff0c\u5c07\u6b64\u4e00\u7d50\u69cb\u7247\u6bb5\u5c0d\u63a5 (manually docked) \u81f3\u6210\u7e96\u7dad\u7d30\u80de\u6d3b\u5316\u86cb\u767d\u7684\u6d3b\u6027\u4f4d\u7f6e\uff0c\u5982\u4e0b\u5716\u3002<\/h3>\n<p><a href=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/fap.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-450\" src=\"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-content\/uploads\/2015\/08\/fap.jpg\" alt=\"fap\" width=\"548\" height=\"561\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<h3>6. \u9019\u4e9b\u7d50\u679c\u767c\u8868\u5728<\/h3>\n<h3>Cleavage-site specificity of prolyl endopeptidase FAP investigated with a full-length protein substrate. Chih-Hsiang Huang, <em>et al<\/em>. J. Biochem. 2011 49: 685-692. [<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=21288888\"><strong>PubMed link<\/strong><\/a>]<\/h3>\n<h3>\u203b\u4ee5\u4e0a\u6240\u63cf\u8ff0\u7684\u904e\u7a0b\u50c5\u50c5\u662f\u8868\u9054\u751f\u7269\u8cc7\u8a0a\u8cc7\u6599\u5eab\u53ca\u5de5\u5177\u7684\u61c9\u7528\u53ca\u5c0d\u5be6\u9a57\u7684\u8f14\u52a9\uff0c\u4e8b\u5be6\u4e0a\uff0c\u5be6\u9a57\u7684\u904e\u7a0b\u662f\u6700\u91cd\u8981\u4e5f\u662f\u6700\u4e3b\u8981\u7684\u8ca2\u737b\u8005\u3002\u82e5\u6709\u8aa4\u5c0e(misleading)\u6216\u5f97\u7f6a(offensive)\u4e4b\u8655\uff0c\u656c\u8acb\u898b\u8ad2\u4e26\u7d66\u4e88\u8cdc\u6559\uff0c\u8b1d\u8b1d\u3002<\/h3>\n<h3><span style=\"color: #0000ff;\"><strong><span style=\"font-family: Thread-00001b6c-Id-00000080;\"><span style=\"font-family: \u65b0\u7d30\u660e\u9ad4;\">\u4ee5\u4e0a\u5167\u5bb9\u611f\u8b1d\u751f\u91ab\u6240\u5b6b\u6176\u59dd\u5c0f\u59d0\u63d0\u4f9b\uff0c\u5e0c\u671b\u5c0d\u60a8\u5011\u6709\u5e6b\u52a9\u3002<\/span><\/span><\/strong><\/span><\/h3>\n<p>&nbsp;<\/p>\n<div class=\"pld-like-dislike-wrap pld-template-1\">\r\n    <div class=\"pld-like-wrap  pld-common-wrap\">\r\n    <a href=\"javascript:void(0)\" class=\"pld-like-trigger pld-like-dislike-trigger  \" title=\"\u8b9a\" data-post-id=\"449\" data-trigger-type=\"like\" data-restriction=\"ip\" data-already-liked=\"0\">\r\n                        <i class=\"fas fa-thumbs-up\"><\/i>\r\n                <\/a>\r\n    <span class=\"pld-like-count-wrap pld-count-wrap\">0    <\/span>\r\n<\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>0<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[16,26],"class_list":["post-449","post","type-post","status-publish","format-standard","hentry","category-bioinformatics","tag-dr-a-cula-lab","tag-fap"],"_links":{"self":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/449","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/comments?post=449"}],"version-history":[{"count":6,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/449\/revisions"}],"predecessor-version":[{"id":461,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/posts\/449\/revisions\/461"}],"wp:attachment":[{"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/media?parent=449"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/categories?post=449"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lsl.sinica.edu.tw\/Blog\/wp-json\/wp\/v2\/tags?post=449"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}