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Development of Primordial germ cells (PGCs) is required for reproduction. During PGC development in mammals, major epigenetic remodeling occurs which is hypothesized to establish an epigenetic landscape for sex-specific germ cell differentiation and gametogenesis...... |
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Co-immunoprecipitation (Co-IP) is a widely used and powerful approach for studying protein-protein interactions in vivo. In a collaboration with Marta Riera and David Caparros-Ruiz from the Centre de Recerca en Agrigenòmica (CRAC) in Barcelona (Spain) initiated by Isabel Vélez-Bermúdez from Wolfgang Schmidt’s lab, a state-of-the-art guide for antibody purification and immobilization followed by immunoprecipitation from plant tissue extracts using magnetic beads has recently been published in the journal STAR Protocols...... |
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Cytochrome (Cyt) b559 is a key component of the photosystem II complex (PSII) for its assembly and proper function. Previous studies have suggested that Cyt b559 has functional roles in the early assembly of PSII and in secondary electron transfer pathways that protect the PSII against photoinhibition. In addition, Cyt b559 in various PSII preparations exhibited multiple different redox potential forms. However, the precise functional roles of Cyt b559 in PSII remain unclear...... |
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Histone deacetylases (HDAs) play an important role in transcriptional regulation of multiple biological processes. In this study, we investigated the function of HDA15 in abscisic acid (ABA) responses. We used immunopurification coupled with mass spectrometry-based proteomics to identify proteins interacting with HDA15 in Arabidopsis...... |
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N-acetylglucosamine (GlcNAc) is the fundamental amino sugar moiety that is essential for various glycosylation events, including protein N-glycosylation. UDP-GlcNAc, an active form of GlcNAc, is synthesized through the hexosamine biosynthetic pathway (HBP) that integrates a number of crucial energy components (fructose-6P, L-Gln, acetyl-CoA and UTP)...... |
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Cytochrome (Cyt) b559 is a key component of the photosystem II complex (PSII) for its assembly and proper function. Previous studies have suggested that Cyt b559 has functional roles in the early assembly of PSII and in secondary electron transfer pathways that protect the PSII against photoinhibition...... |
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Dr. Ming-Hsiun Hsieh’s laboratory reports that the Arabidopsis glutamine synthetase2 mutants grow normally under air or high CO2 (Fig. 1), challenging the textbook that chloroplastic GS2 is the primary enzyme to assimilate NH4+ derived from photorespiration. The paradigm that the chloroplastic GS/GOGAT cycle is the primary route for NH4+ assimilation is questionable, at least in the reference plant Arabidopsis thaliana...... |
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To elucidate this challenge, a team led by Dr. Ming-Che Shih at Agricultural Biotechnology Research Center, Academia Sinica, unveiled that General Control Nonderepressible 2 (GCN2) and Ethylene Insensitive 2 (EIN2) are activated to modulate the translational repression through detection of passively entrapped ethylene under submergence (15 min) in Arabidopsis. Both GCN2 and EIN2 can regulate transcript levels and translation efficiency of specific mRNAs during submergence...... |
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the research team of Chuan Ku at the Institute of Plant and Microbial Biology, Academia Sinica, investigated the evolutionary history of mitochondrial, plastid, and nuclear genomes in coccolithophores, calcifying microalgae that are commonly found across global oceans. In the nuclear genome phylogeny, the most abundant species, Emiliania huxleyi, and several closely related species are separated into clades with distinct morphology or distribution patterns. However, such relationships are absent in the mitochondrial and plastid phylogenies, which group strains from different species into the same clades and show branching patterns highly incongruent with those in the nuclear phylogeny. Furthermore, the two organellar phylogenies differ substantially from each other, which has not been observed in previous studies on plants...... |
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